Song missed the goalless draw with Sunderland at the emirates Stadium on Saturday with a knee injury sustained in the Carling Cup final.
After undergoing a fitness test on Sunday, the Cameroon international has been ruled out and will not travel with the squad to the Camp Nou for the last-16 second-leg clash with the Spanish champions.
Song joins several other first-team players on the injured list for the Gunners, ho have striker Robin van Persie, winger Theo Walcott and long-term absentee Thomas Vermaelen also injured.
However, there is hope that captain Cesc Fabregas will be able to recover from a hamstring injury in tie to face the club he left to join Arsenal as a 16-year-old.
Arsenal take a 2-1 aggregate lead to Catalonia, but insisted that his team would not be aiming to sit on that slender lead, and were not equipped to do so even if they wanted to.
"We'll make some changes for Tuesday, that's for sure. We need some fresh legs for the Nou Camp because we'll have to run a lot there," said Wenger.
"We've not got enough security just to sit back and defend; you can't go to Barcelona and play for a 0-0. Anyway, it's not the personality of my team. This game has been hanging a bit over the heads of my players but our focus is on it now."
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